Search results for "physical environment"
showing 10 items of 17 documents
Chromatin organization regulates viral egress dynamics.
2017
Various types of DNA viruses are known to elicit the formation of a large nuclear viral replication compartment and marginalization of the cell chromatin. We used three-dimensional soft x-ray tomography, confocal and electron microscopy, combined with numerical modelling of capsid diffusion to analyse the molecular organization of chromatin in herpes simplex virus 1 infection and its effect on the transport of progeny viral capsids to the nuclear envelope. Our data showed that the formation of the viral replication compartment at late infection resulted in the enrichment of heterochromatin in the nuclear periphery accompanied by the compaction of chromatin. Random walk modelling of herpes s…
Permeability changes of integrin-containing multivesicular structures triggered by picornavirus entry.
2014
Cellular uptake of clustered α2β1-integrin induces the formation of membrane compartments that subsequently mature into a multivesicular body (MVB). Enhanced internalization mediated by clustered integrins was observed upon infection by the picornavirus echovirus 1 (EVI). We elucidated the structural features of virus-induced MVBs (vMVBs) in comparison to antibody-induced control MVBs (mock infection) by means of high-pressure cryo fixation of cells followed by immuno electron tomography during early entry of the virus. Three-dimensional tomograms revealed a marked increase in the size and complexity of these vMVBs and the intraluminal vesicles (ILVs) at 2 and 3.5 hours post infection (p.i.…
Global, regional, and national age-sex specific all-cause and cause-specific mortality for 240 causes of death, 1990-2013: a systematic analysis for …
2015
Background Up-to-date evidence on levels and trends for age-sex-specific all-cause and cause-specific mortality is essential for the formation of global, regional, and national health policies. In the Global Burden of Disease Study 2013 (GBD 2013) we estimated yearly deaths for 188 countries between 1990, and 2013. We used the results to assess whether there is epidemiological convergence across countries. Methods We estimated age-sex-specific all-cause mortality using the GBD 2010 methods with some refinements to improve accuracy applied to an updated database of vital registration, survey, and census data. We generally estimated cause of death as in the GBD 2010. Key improvements included…
Global, regional, and national incidence, prevalence, and years lived with disability for 301 acute and chronic diseases and injuries in 188 countrie…
2015
Summary Background Up-to-date evidence about levels and trends in disease and injury incidence, prevalence, and years lived with disability (YLDs) is an essential input into global, regional, and national health policies. In the Global Burden of Disease Study 2013 (GBD 2013), we estimated these quantities for acute and chronic diseases and injuries for 188 countries between 1990 and 2013. Methods Estimates were calculated for disease and injury incidence, prevalence, and YLDs using GBD 2010 methods with some important refinements. Results for incidence of acute disorders and prevalence of chronic disorders are new additions to the analysis. Key improvements include expansion to the cause an…
Early Life Exposure to Perfluoroalkyl Substances (PFAS) and ADHD: A Meta-Analysis of Nine European Population-Based Studies
2020
Introduction: To date, the evidence for an association between perfluoroalkyl substances (PFAS) exposure and attention deficit and hyperactivity disorder (ADHD) is inconclusive. Objective: We investigated the association between early life exposure to perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA), and ADHD in a collaborative study including nine European population-based studies, encompassing 4,826 mother-child pairs. Methods: Concentrations of PFOS and PFOA were measured in maternal serum/plasma during pregnancy, or in breast milk, with different timing of sample collection in each cohort. We used a validated pharmacokinetic model of pregnancy and lactation to estimate…
Different neighborhood walkability indexes for active commuting to school are necessary for urban and rural children and adolescents
2020
This work was supported by the Spanish Ministry of Economy, Industry and Competitiveness and the European Regional Development Fund [grant no. DEP2016-75598-R, MINECO/FEDER, UE]. Additionally, this study takes place thanks to the funding from the University of Granada Plan Propio de Investigacion 2016 -Excellence actions: Unit of Excellence on Exercise and Health (UCEES) -and the Junta de Andalucia, Consejeria de Conocimiento, Investigacion y Universidades, European Regional Development Fund [grant no. SOMM17/6107/UGR]. The funding body had no role in the study design or collection, analysis, or interpretation of the data or in writing the manuscript.
Senior Housing as a Living Environment That Supports Well-Being in Old Age
2020
Background: In different parts of the world new models of senior housing have rapidly appeared, which indicates that existing housing and care models are not fulfilling the hopes and needs of current and new generations of older people. Material and Methods: This qualitative study focuses on one type of communal senior housing complex located in a mid-sized town in Central Finland. The complex was designed to have accessible low-maintenance apartments and common spaces, and to be near easily accessible green spaces, amenities, services, and public transport. The complex has a part-time community coordinator. The minimum age limit is set at 55 years. The data consists of 36 qualitative inter…
Exceptional Heterogeneity in Viral Evolutionary Dynamics Characterises Chronic Hepatitis C Virus Infection.
2016
The treatment of HCV infection has seen significant progress, particularly since the approval of new direct-acting antiviral drugs. However these clinical achievements have been made despite an incomplete understanding of HCV replication and within-host evolution, especially compared with HIV-1. Here, we undertake a comprehensive analysis of HCV within-host evolution during chronic infection by investigating over 4000 viral sequences sampled longitudinally from 15 HCV-infected patients. We compare our HCV results to those from a well-studied HIV-1 cohort, revealing key differences in the evolutionary behaviour of these two chronic-infecting pathogens. Notably, we find an exceptional level o…
Attaccamento ai luoghi e prospettive abitative in un campione di utenti in comunità terapeutiche assistite psichiatriche
2011
Aim: The study aimed to inquire place attachment bonds in a sample of psychiatric patient hosted in Residential Mental Health Facilities of Palermo. Satisfaction for current accommodation in the Facility, reasons for place preference and housing perspectives were explored. Methods: A questionnaire consisting in 31 open questions was administered to 39 subjects living in three Residential Mental Health Facilities of Health Department of Palermo. Results: Own room and terraces with plats are the patients' favourite places in the Facilities. During the first month of stay, these places satisfy need for retirement and distraction, while in the following period they become spaces where meet peop…
Pyöräilytaidon oppimista selittävät yksilö-, ympäristö- ja tehtävätason tekijät 1950-2010-luvuilla
2021
Pyöräily on yksi suomalaisten yleisimmistä liikkumismuodoista. Yhteiskunnan tasolla pyöräilyn merkityksissä korostuvat väestön arkiliikunnan lisääminen ja kestävän liikenteen edistäminen. Suomalaislapsille pyöräilytaito mahdollistaa merkittävän itsenäisen liikkumisen tavan ja edesauttaa elinpiirin laajentumista. Pyöräilytaidon oppimiseen liittyvistä tekijöistä tiedetään kuitenkin hyvin vähän. Tämä tieto olisi tärkeää, jotta mahdollisimman monelle voitaisiin jo varhaisella iällä tarjota mahdollisuus omaksua pyöräilyn kautta fyysisesti aktiivinen ja ekologisesti kestävä elämäntapa. Tämän tutkimuksen tarkoitus oli selvittää, mitkä yksilö-, ympäristö- ja tehtävätekijät selittävät pyöräilytaidon…